COL1A1 Rabbit pAb A16891 from ABclonal Technology

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Journal of Translational Medicine
September 23 2022

Fig 1: Overexpression of MIR99AHG suppresses EMT and fibrosis. A Analysis of LncRNA MIR99AHG expression in LUAD and normal lung tissues using the GEPIA database. B Analysis of the relationship between LncRNA MIR99AHG expression and survival of LUAD patients using the Kaplan–Meier plotter. C RT-qPCR detection of LncRNA MIR99AHG expression. D RT-qPCR to detect the stable overexpression efficiency of LncRNA MIR99AHG. E RT-qPCR to detect the expression of E-Cadherin, Vimentin, ACTA2 and COL1A1 after overexpression of LncRNA MIR99AHG. F, G The protein expression of TGF-β1, E-Cadherin, Vimentin, ACTA2 and COL1A1 was detected by WB and statistically quantified by ImageJ software. H, I After overexpression of LncRNA MIR99AHG, cell migration was monitored by wound scratch assay. Statistical data are shown. J, K After overexpression of LncRNA MIR99AHG, cell invasion was measured by invasion assay. Cell invasion was determined by invasion assay and counted by ImageJ counting. L, M Immunofluorescence assay was performed to detect the expression of fibrosis-related proteins after overexpression of LncRNA MIR99AHG. Statistical data are shown. N Subcutaneous tumorigenesis demonstration in nude mouse. O Tumor weights were represented as the means of tumor weights ± SD. P RT-qPCR to confirm the overexpression of LncRNA MIR99AHG in tumor tissues. Q, R WB to detect the expression of TGF-β1, E-Cadherin, Vimentin, ACTA2 and COL1A1 in MIR99AHG stabled overexpression tumor tissues. Statistical data are shown. S The tumor sections underwent IHC staining and H&E staining. *P < 0.05, **P < 0.01
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Stem Cell Research & Therapy
December 01 2021

Fig 2: Influences of Morusin on osteogenic differentiation of BMSCs. a Detection of the expression of osteo-specific genes (Col1a1, Runx2, and Bsp) at days 3, 5, and 7 of osteogenic differentiation via quantitative real-time PCR. We normalized the gene expression levels to GAPDH. b Relative protein expression of osteo-specific genes (COL1A1 and RUNX2) at days 3, 5, and 7 of osteogenic differentiation. The protein expression level is the gray value ratio of the target protein to GAPDH. c ALP staining of the osteogenic BMSCs. Scale bar, 250 μm. d Alizarin red staining of the osteogenic BMSCs. Scale bar, 250 μm. BMSCs, bone marrow mesenchymal stem cells; RUNX2, runt-related transcription factor 2; COL1A1, collagen type I alpha 1; BSP, bone sialoprotein. We repeated the independent experiment for three times. Means ± SEM. *P < 0.05 and **P < 0.01 in comparison to the control group. “ns” refers to no statistical significance compared with the control group
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Cellular and Molecular Gastroenterology and Hepatology
February 22 2022

Fig 3: Heg-deficiency in liver ECs do not affect liver function under unchallenged condition.A, Serum level of liver enzymes (ALP/ALT/AST), ALB, and TBIL show no significant difference between control and Lyve1-Cre;Hegfl/fl mice at 12 weeks. B, H&E staining, sirius red staining, and immunostaining of Desmin in control and Lyve1-Cre;Hegfl/fl liver sections. C, mRNA levels of liver injury markers (Col1a1, Acta2, Desmin, Ddr2, and Tgfβ), and (D) mRNA level of macrophage markers (F4/80, Il1β, and Il6). Data are presented as mean ± SD using unpaired Student t test. Scale bars represent 50 μm.
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Stem Cell Research & Therapy
December 01 2021

Fig 4: The impacts of Morusin on proliferation and osteogenic differentiation of BMSCs. a The chemical structure of Morusin. b Detection of the proliferation toxicity of Morusin on BMSCs via MTT assay. BMSCs were cultured in osteogenic growth medium (OGM) with diverse concentrations of Morusin for 3, 5, 7, and 14 days. c–f mRNA level of marker genes for osteogenesis. Quantitative real-time PCR analysis of gene expression of osteogenic differentiation markers. We incubated BMSCs with Morusin of diverse concentrations (0–50 μM) for 3 days to observe the impact of Morusin on osteogenic differentiation. BMSCs, bone marrow mesenchymal stem cells; RUNX2, runt-related transcription factor 2; COL1A1, collagen type I alpha 1; BSP, bone sialoprotein; OCN, osteocalcin. We repeated the independent experiment for three times, with similar results achieved each time. Means ± SEM. *P < 0.05 and **P < 0.01 in comparison to the control group
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Journal of Translational Medicine
September 23 2022

Fig 5: USP4 deubiquitination maintains ACE2 stability and thus inhibits EMT and fibrosis in LUAD. A Analysis of USP4 expression in LUAD and normal lung tissues using the GEPIA database. B Analysis of USP4 expression in relation to OS of LUAD patients using Kaplan–Meier plotter. C Detection of USP4 mRNA expression in BEAS-2B and A549 by RT-qPCR. D, E Detection of USP4 protein expression in BEAS-2B and A549 by WB and statistical quantification by ImageJ software. F RT-qPCR detection of USP4 mRNA expression levels after overexpression of USP4. G, H WB detection of USP4 protein expression levels after overexpression of USP4 and statistical quantification by ImageJ software. I Overexpression of USP4 followed by RT-qPCR for EMT and fibrosis marker expression. J, K The protein expressions of TGF-β1, E-cadherin, vimentin, ACTA2 and COL1A1 were detected by WB after overexpression of USP4. Statistical quantification by ImageJ software. L, M After overexpression of USP4, cell migration was monitored by wound scratch assay. N, O After the expression of USP4, cell invasion was determined by invasion assay. And counted statistically by ImageJ. P, Q After overexpression of USP4, an immunofluorescence assay was performed to detect the expression of fibrosis-associated proteins. Statistical data are shown. R Detection of ACE2 mRNA expression by RT-qPCR after overexpression of USP4. S, T Detection of ACE2 protein expression by WB after overexpression of USP4 and quantified by ImageJ software statistics. U Functional enrichment analysis of USP4 by Metascap website. V Analysis of ubiquitinated degradation of ACE2 after overexpression of USP4 by Co-IP assay

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COL1A1 Rabbit pAb

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The COL1A1 Rabbit pAb Antibody from ABclonal Technology is a Rabbit Polyclonal antibody to COL1A1. This antibody recognizes Human, Mouse, and Rat antigen. The COL1A1 Rabbit pAb Antibody has been validated for the following applications: Precipitation, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blot.

Product Specs
ItemCOL1A1 Rabbit pAb
CompanyABclonal Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberA16891
Antibody TypePrimary Antibody
ClonalityPolyclonal
ReactivityHuman, Mouse, Rat
FormatStore at -20℃. Avoid freeze / thaw cycles.:Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3
ApplicationsWB, IHC, IF, IP
IsotypeIgG
ImmunogenA synthetic peptide of human COL1A1
ModificationsUnmodified
TargetCOL1A1
Conjugate/TagUnconjugated
HostRabbit
Quantity100 µL
PurityAffinity purification
NCBI Full Gene Namecollagen type I alpha 1 chain
NCBI Gene AliasesEDSC, CAFYD, EDSARTH1, OI4, OI3, OI2, OI1
Concentration0.495 mg/ml

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ABclonal Technology

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Phone: 888.754.5670
Int’l: +1857.259.4898

Email: service@abclonal.com

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Website: https://abclonal.com/

(1) Liquid platelet-rich fibrin produced via horizontal centrifugation decreases the inflammatory response and promotes chondrocyte regeneration in vitroFront Bioeng BiotechnolDecember 7, 2023Huimin Li, Ting Xia, Hao Zeng, Yun Qiu, Yan Wei, Yihong Cheng, Yulan Wang, Xiaoxin Zhang, Jin Ke, Richard Miron, et al.4°C: rabbit anti-Aggrecan (1:1000, Proteintech, 13880-1-AP), rabbit anti-Col2a1 (1:1000, Abclonal, A16891), rabbit anti-Adamts5 (1:1000, abcam, ab41037), rabbit anti-Il-6 (1:1000, Proteintech, 21865-1-AP),

(2) Autophagosomes Defeat Ferroptosis by Decreasing Generation and Increasing Discharge of Free Fe(2+) in Skin Repair Cells to Accelerate Diabetic Wound HealingAdvanced science (Weinheim, Baden-Wurttemberg, Germany)September 1, 2023Cui S, Liu X, Liu Y, Hu W, Ma K, Huang Q, Chu Z, Tian L, Meng S, Su J, et al.that, the sections were incubated with anti‐CD31 (1:100, Abcam, ab281586), COL1A1 (1:100, Abclonal, A16891), α‐SMA (1:100, Abclonal, A17910), and FTH1 (1:200, CST, 4393) at 4 °C for 12 h. Statistical Analysis GraphPad

(3) CircItgb5 promotes synthetic phenotype of pulmonary artery smooth muscle cells via interacting with miR-96-5p and Uba1 in monocrotaline-induced pulmonary arterial hypertensionRespiratory ResearchJune 21, 2023Hua Su, Huiqi Zhu, Sihao Wang, Yeping Li, Chao Yan, Jiaoyan Wang, Kejing Yingincubated with the primary antibodies for 18 h at 4 °C. Primary antibodies included COL1A1 (1:1000, A16891, ABclonal, USA), SM22 (1:1000, A6760, ABclonal, USA), AGO2 (1:1000, C34C6, CST, USA), mTOR (1:1000,

(4) Blockage of Osteopontin‐Integrin β3 Signaling in Infrapatellar Fat Pad Attenuates Osteoarthritis in MiceAdvanced ScienceMay 23, 2023Bingyang Dai, Yuwei Zhu, Xu Li, Zuru Liang, Shunxiang Xu, Shian Zhang, Zhe Zhang, Shanshan Bai, Wenxue Tong, Mingde Cao, et al.Integrin β3 (Thermo Fisher Scientific, USA, cat # PA585926, 1:100), and COL1A1 (Abclonal, USA, cat# A16891, 1:50) diluted in 1% BSA buffer (1% BSA in PBS) overnight at 4 °C. Subsequently, sections were incubated

(5) Exosomes Derived from Adipose Tissue-Derived Mesenchymal Stromal Cells Prevent Medication-Related Osteonecrosis of the Jaw through IL-1RAInternational Journal of Molecular SciencesMay 12, 2023Yi Zheng, Xian Dong, Xinyu Wang, Jie Wang, Shuo Chen, Yang He, Jingang An, Linhai He, Yi Zhangby 5% normal goat serum for 30 min at RT, and incubated at 4 °C overnight with antibodies, COL1A1 (A16891, Abclonal, Wuhan, China), IL-1RA (ab124962, Abcam, Cambridge, UK), and FN (A16678, Abclonal, China).

(6) OTUB1 promotes osteoblastic bone formation through stabilizing FGFR2Signal Transduction and Targeted TherapyApril 7, 2023Qiong Zhu, Yesheng Fu, Chun-Ping Cui, Yi Ding, Zhikang Deng, Chao Ning, Fan Hu, Chen Qiu, Biyue Yu, Xuemei Zhou, et al.anti-Multi Ubiquitin (D058-3) antibodies were purchased from MBL. Anti-β-actin (AC026) and anti-COL1A1 (A16891) antibodies were purchased from Abclonal. Peroxidase-AffiniPure goat anti-rabbit IgG (111-035-003) and

(7) DAB2 promotes pulmonary fibrosis and may act as an intermediate between IGF‑1R and PI3K/AKT signaling pathwaysExperimental and Therapeutic MedicineMarch 10, 2023Chun-Lian Liang, Xiu-Li Li, Xiao-Juan Quan, Lin ZhangLtd.","1:1,000",4˚C overnight DAB2 antibody,bs-5999R,BIOSS,"1:1,000",4˚C overnight Collagen I antibody,A16891,"ABclonal Biotech Co., Ltd.","1:1,000",4˚C overnight Collagen Ⅳ antibody,AF0510,"Affinity Biosciences,

(8) HNRNPC, a predictor of prognosis and immunotherapy response based on bioinformatics analysis, is related to proliferation and invasion of NSCLC cellsRespiratory ResearchDecember 19, 2022Zhuoyu Gu, Yang Yang, Qian Ma, Hui Wang, Song Zhao, Yu Qi, Yixin Liprimary antibodies used were as follows: HNRNPC (Abclonal, #A19137, 1:100), Collagen I (Abclonal, #A16891, 1:100), CD31 (BD, #550274, 1:200), CD4 (Abcam, #ab183685, 1:100) and CD8 (Abcam, #ab217344, 1:200). GEPIA,

(9) Rubus chingii Hu. unripe fruits extract ameliorates carbon tetrachloride-induced liver fibrosis and improves the associated gut microbiota imbalanceChinese MedicineDecember 1, 2022Jianjun Wu, Dingqi Zhang, Bo Zhu, Siqi Wang, Yongbin Xu, Congcong Zhang, Hailing Yang, Shunchun Wang, Ping Liu, Luping Qin, et al.and Smad4 (Cat#ab40759) were obtained from Abcam, Inc. (Cambridge, UK). Type I collagen (Col1A1, Cat#A16891) was acquired from ABclonal Biotechnology Co., Ltd. (Wuhan, China). PVDF membrane was obtained from

(10) Extracellular matrix derived from Wharton’s Jelly-derived mesenchymal stem cells promotes angiogenesis via integrin αVβ3/c-Myc/P300/VEGFStem Cell Research & TherapyDecember 1, 2022Beilei Ma, Tengkai Wang, Juan Li, Qian Wang(A11131), phospho-FAK-Y397 (p-FAK) (AP0302), P38 (A14401), phospho-P38 (AP0055), Collagen I (COL I) (A16891) and cellular (c)-Myc (A1309) were obtained by Abclonal Inc. (China). The antibody of P300 (sc-48343)

(11) Inhibition of Wdr5 Attenuates Ang-II-Induced Fibroblast-to-Myofibroblast Transition in Cardiac Fibrosis by Regulating Mdm2/P53/P21 PathwayBiomoleculesOctober 27, 2022Jiali Yuan, Hong Peng, Binfeng Mo, Chengye Yin, Guojian Fang, Yingze Li, Yuepeng Wang, Renhua Chen, Qunshan Wangab178410), H3K4me3 (1:1500, Abcam, ab8580), α-SMA (1:3000, Abcam, ab6276), Collagen I (1:800, Abclonal, A16891), P53 (1:1000, Abclonal, A5761), Mdm2 (1:1000, Abclonal, A0345), P21 (1:800, Abcam, ab109199), and α-tubulin

(12) MIR99AHG inhibits EMT in pulmonary fibrosis via the miR-136-5p/USP4/ACE2 axisJournal of Translational MedicineSeptember 23, 2022Jun Wang et al.AC002, ABclonal, Wuhan, China), Anti-USP4 (1:1000, A20005, ABclonal, Wuhan, China), Anti-COL1A1 (1:1000, A16891, ABclonal, Wuhan, China), Anti-ACTA2 (1:1000, A1011, ABclonal, Wuhan, China), Anti-Vimentin (1:1000,

(13) Enhancement of Tendon Repair Using Tendon-Derived Stem Cells in Small Intestinal Submucosa via M2 Macrophage PolarizationCellsSeptember 5, 2022Mao X, Yao L, Li M, Zhang X, Weng B, Zhu W, Ni R, Chen K, Yi L, Zhao J, et al.paraformaldehyde (P8430, Sinopharm Group, Shanghai, China) for 20 min, stained with anti-rabbit COL1A1 (A16891, Abclonal, Wuhan, China) and DAPI (C0065, Solarbio, Beijing, China), and then counted in five randomly

(14) ShenKang Injection Attenuates Renal Fibrosis by Inhibiting EMT and Regulating the Wnt/β-Catenin PathwayEvidence-Based Complementary and Alternative MedicineJune 28, 2022Hui-Ting Wei, Yuan Xu, Xiao-Yang Tan, Hao-Yue Jing, Yue-Rong MaProtein,Primary antibody,Concentration Collagen I,Abclonal; A16891,1 : 500 FN,Abcam; ab268021,1 : 2000 α-SMA,Abclonal; A17910,1 : 500 E-cadherin,Abcam; ab231303,1 : 1000

(15) Liver Endothelial Heg Regulates Vascular/Biliary Network Patterning and Metabolic Zonation Via Wnt SignalingCellular and Molecular Gastroenterology and HepatologyFebruary 22, 2022Shichao Zhu et al.primary antibodies were used: anti-desmin (Abcam, ab15200, 1:300 dilution), anti-Col1a1 (ABclonal, A16891, 1:300 dilution), anti-Ck19 (Abcam, ab133496, 1:300 dilution), anti-GS (Abcam, ab49873, 1:5000 dilution),

(16) HNRNPK/CLCN3 axis facilitates the progression of LUAD through CAF-tumor interactionInternational journal of biological sciencesJanuary 1, 2022Li Y, Yang Y, Ma Q, Cheng H, Wang H, Ma C, Li F, Zhao S, Li X, Qi Y, et al.#10176-2-AP, 1:1000), p-AKT (CST, #4060, 1:1000), α-SMA (Abclonal, #A17910, 1:1000), COL1A1 (Abclonal, #A16891, 1:1000), FAP (Abclonal, #A6349, 1:1000), β-Actin (Proteintech, #20536-1-AP, 1:2000) and Histone H3

(17) Morusin induces osteogenic differentiation of bone marrow mesenchymal stem cells by canonical Wnt/β-catenin pathway and prevents bone loss in an ovariectomized rat modelStem Cell Research & TherapyDecember 1, 2021Ming Chen, Hui Han, Siqi Zhou, Yinxian Wen, Liaobin Chensc-390351) was ordered from Santa Cruz Biotechnology Co. (Dallas, USA). The collagen type I alpha 1(COL1A1, A16891), phosphorylated β-catenin (p-β-catenin, AP0579), and β-catenin (A19657) antibody was obtained from

(18) Changes in aortic collagen in β-aminopropionitrile-induced acute aortic dissectionAnnals of translational medicineOctober 1, 2021Zhou H, Ren Y, Xiao J, He J, Zhang Y, Qiu Z, Huang Q, Hu Y, Chen L.monoclonal anti-collagen I antibody (ab260043) were purchased from Abcam (UK); rabbit anti-COL1α1 (A16891), COL1α2 (A16699), and COL3α1 (A3795) were purchased from Abclonal (China); rabbit anti-collagen III

(19) Combination CTLA-4 immunoglobulin treatment and ultrasound microbubble-mediated exposure improve renal function in a rat model of diabetic nephropathyAging (Albany NY)March 31, 2021Liang Wang, Pengfei Wang, Xiuyun Li, Yanyan Dong, Senmin Wu, Maosheng Xu, Xiu Chen, Shijia Wang, Chao Zheng, Chunpeng Zouanti-CD34 (ab8158, Abcam), anti-IL6 (ab208113, Abcam), anti-Fibronectin (ab268021, Abcam), anti-Collagen I (A16891, Abclonal), anti-Talin 1 (ab71333, Abcam), anti-Paxillin (ab32084, Abcam), anti-Integrin alpha 3 (AF5182,

(20) High-Salt Attenuates the Efficacy of Dapagliflozin in Tubular Protection by Impairing Fatty Acid Metabolism in Diabetic Kidney DiseaseFrontiers in pharmacologyJanuary 1, 2021Zou M, Chen Y, Zheng Z, Sheng S, Jia Y, Wang X, Ren S, Yang Y, Li X, Dong W, et al.anti-FN (1:2000, 66042-1-Ig, Proteintech Group, Inc. Rosement, United States), anti-COL-1 (1:1,000, A16891, ABclonal, Wuhan, China), anti-αSMA (1:500, A7248, ABclonal), anti-TGF-β1 (1:500, A18692, Santa Cruz,

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Journal of Translational Medicine

Stem Cell Research & Therapy

Cellular and Molecular Gastroenterology and Hepatology

Stem Cell Research & Therapy

Journal of Translational Medicine

COL1A1 Rabbit pAb from ABclonal Technology

ABclonal Technology

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COL1A1 Rabbit pAb from ABclonal Technology

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